| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2024-12-25 |
| タイトル |
|
|
タイトル |
SDS- ポリアクリルアミドゲル電気泳動（SDS-PAGE）におけるSDS が与えるリン酸化タンパクの相対移動度への影響 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Effect of SDS on migration index of phosphorylated proteins in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.51095/kiyo.59.02 |
|
ID登録タイプ |
JaLC |
| 著者 |
木下, 英司
面出, 鈴音
加藤, 舞
塩田, ゆきの
西田, りん
矢田, 明日香
山田, 梨緒菜
木下, 恵美子
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
A variety of protein-targeted studies are being conducted to gain a fundamental understanding of living systems and to elucidate the mechanisms of disease pathogenesis. In such life science research fields, SDS-PAGE is widely used for protein separation and analysis. In this study, several proteins were analyzed by SDS-PAGE to better understand the characteristics and properties of the relationship between the molecular weight and electrophoretic migration index of proteins under various conditions. We also investigated the effect of SDS on the migration index of phosphorylated proteins, focusing on the phenomenon that the migration rate of phosphorylated proteins in SDS-PAGE is slower than that of dephosphorylated counterparts. The results revealed that the relative mobility of proteins is affected by the concentrations of the resolving gel matrix and cross-linking agent and may be also significantly affected by the addition of phosphate groups, one of the post-translational modifications. We thus concluded that it is necessary to examine the proteins under various SDS-PAGE conditions, paying close attention to these points, depending on the protein to be analyzed. Furthermore, the effect of SDS on the migration index was examined by changing the concentration of SDS in the electrophoresis sample buffer and the running buffer. The results showed that electrophoresis was inhibited to a greater extent at lower SDS concentrations in both buffers, and the relative mobility of the phosphorylated proteins was significantly smaller, but the correlation with molecular weight was also greatly disturbed. Finally, it was concluded that the influence of SDS on SDS-PAGE is enormous, and that it is extremely difficult to find the cause of the small migration index of phosphorylated proteins in SDS-PAGE by varying the concentration of SDS in the electrophoresis sample buffer and the running buffer. |
|
言語 |
en |
| bibliographic_information |
ja : 広島文教大学紀要
en : Bulletin of Hiroshima Bunkyo University
巻 59,
p. 11-22,
発行日 2024-12-20
|
| 出版者 |
|
|
出版者 |
広島文教大学 |
|
言語 |
ja |
| item_10002_source_id_9 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
24351660 |
| item_10002_source_id_11 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12881318 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
kiyo59.011-022.pdf (1.5 MB)
